CIC Panels

Note: The CIC would especially like to thank the Vanderbilt Center for Immunobiology (VCI) for supporting the development and testing of the first mouse mass cytometry CIC Panel, CIC-005 – Mouse Systems Immune Monitoring (SIM).

The CIC provides mass cytometry staining only with the following validated CIC Panels.

  1. CIC-001Human B cell emphasis28 targets
    1. Targets are all human and include: CD19, CD5, CD38, CD4, IgD, CD20, CD16, CD25, CD43, Ig lambda, CD21, CD62L, CD45, CD36, CD22, Ig kappa, CD79B, CXCR3, CD40, CD27, CD8, CD24, CD3, CXCR5, IgM, HLA-DR, CD14, CD56.
    2. Open channels (7): 141, 156, 158, 161, 164, 166, and 173.
  2. CIC-002Human T cell emphasis (A)34 targets
    1. Targets are all human and include: ICOS, CD19, TIM3, CCR5, CD4, CD8a, CD20, CD16, CCR4, LAG3, CD14, TCRgd, CD45RA, CD45, CD27, CXCR3, CD33, CCR7, CD28, CD69, HLA-DR, CD45RO, CD40, CD44, CD38, CD127, CD25, CD3, CXCR5, CD57, CXCR4, PD-1, PD-L1, CD56.
    2. Open channel (1): 161
  3. CIC-003Human T cell emphasis (B) – 32 targets
    1. Targets are all human and include: CCR6, CD19, CD45RA, CD69, CD4, CD20, CD16, CCR4, LAG3, ICOS, TCRgd, CD62L, TIM3, CD27, CD45, CD33, CCR7, CD28, CD8, CXCR3, CD95, CD45RO, CD44, CD38, CD127, CD25, CD3, CXCR5, CD57, HLA-DR, PD-1, CD56
    2. Open channels (3): 146, 161, 173
  4. CIC-004Human myeloid cell emphasis – 33 targets
    1. Targets are all human and include: CD19, CD117, CD11b, CD4, CD64, CD36, CD164, CCR2, p-STAT5, CD123, CD13, p-STAT1, CD45, CD45RA, CD86, p-STAT3, CD11c, CD14, CD32, S100A9, HLA-DR, CD206, CD16, CD120a, CCR7, CD8, CD33, CD3, CD68, CXCR4, PD-1, PD-L1, CD127
    2. Open channels (2): 141, 172

Currently undergoing validation:

  1. CIC-005Mouse Systems Immune Monitoring (SIM) – 26 targets
    1. Targets are all mouse and include: Ly6GC, CD11c, IL-5, IL-2, CD69, F4/80, CD45, CD11b, CD19, CD25, CD3e, TER-119, IL-10, CD62L, iNOS, TNFa, IFNg, IL-4, IL-6, CD8a, TCRb, NK1.1, CD44, CD4, IL-17a, B220
    2. Open channels (9): 150, 153, 155, 156, 159, 163, 164, 173, 175
  2. CIC-006Mouse spleen B cell emphasis – 24 targets
    1. Targets are all mouse and include: CXCR5, ICOS, CD4, CD43, CD45, CD11b, CD19, IgD, IgM, CD3e, CD8a, CD48, CD9, CD23, CD5, CD40, CD1d, CD54, CD21, CD44, CD86, I-A\I-E, CD38, B220
    2. Open channels (11): 141, 144, 155, 156, 164, 165, 166, 167, 169, 170, 173

Custom conjugated reagents may be used in CIC panels following successful Antibody Titration & Validation.

CIC Panels typically use mass cytometry channels from 141 through 176 for metal conjugated antibodies (141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176).