Laboratory of Suman R. Das, Ph.D.

There are three primary aspects to research in the Das lab:

VIRAL GENOMICS

Our lab utilizes high-throughput, next-generation sequencing technologies to assemble complete viral genomes. These include H1N1 influenza in swine, enterovirus 68 from the 2014 outbreak, enterovirus A71 in Vietnam, and respiratory syncytial virus (RSV) from diverse regions worldwide. By analyzing this data, we conduct phylodynamic analyses to comprehend viral evolutionary dynamics. This research not only enhances our understanding of fundamental viral life cycles but also aids in monitoring outbreaks for future prevention strategies. Specifically, we employ deep sequencing to investigate viral transmission and the emergence of drug resistance.

 

    VIRUS-HOST MICROBIOME INTERACTIONS

Emerging evidence indicates a significant interaction between pathogenic viruses and the microbiome, which can either mitigate or worsen disease outcomes. Our research focuses on elucidating virus-induced alterations in the host microbiome by employing amplicon-based and metagenomic sequencing techniques in clinical cohorts (INSPIRE) as well as model organisms such as mice and cotton rats. By doing so, we aim to uncover the impact of the microbiome during acute viral infections, specifically respiratory syncytial virus (RSV) and influenza, and its influence on long-term health outcomes such as wheezing and asthma in infants.

 

VIRAL EVOLUTION MODELING

 Our research involves both in vitro and in vivo modeling of influenza and other RNA viruses to gain insights into antigenic drift. To achieve this, we employ a combination of mathematical, bioinformatic, and synthetic genomic approaches. By studying the evolutionary dynamics of influenza, we aim to identify optimal seasonal vaccine strains. Additionally, we have developed laboratory protocols that enable us to analyze antibody responses to specific viral strains in cell culture.