Congratulations to Nalin Leelatian and co-authors on this publication!
Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA*, Irish JM*. Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis. Current Protocols in Molecular Biology 2017 Apr 3;118:25C.1.1-25C.1.23. PMC5518778. Pubmed. DOI.
► This peer-reviewed protocol for isolation and mass cytometry analysis of single cells from human tissues and tumors was developed with Rebecca Ihrie’s lab and is based on Leelatian and Doxie et al., Cytometry B 2017 published October 2016 entitled Single cell analysis of human tissues and solid tumors with mass cytometry. Since publication, this protocol has been validated for most human and mouse tissues. The protocol describes tumor and tissue disaggregation into single cells and is compatible with single cell approaches like mass cytometry, cell sorting, and single cell RNA-seq.