Congrats to Mikael Roussel and Todd Bartkowiak on their publication of a review and protocol for polarization of human monocytes and assessment by mass cytometry.
- Roussel M, Bartkowiak T, Irish JM. Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry. Methods in Molecular Biology 2019 1989:217-226. doi: 10.1007/978-1-4939-9454-0_14.
► This peer-reviewed publication provides a detailed protocol for 1) polarizing human monocytes into different subtypes and 2) high dimensional analysis of the resulting cells by mass cytometry. Polarized cell types include M1 (M_LPS, M_IFNg), M2 (M_IL-4, M_IL-10), and myeloid derived suppressor cells (MDSCs). Based on Roussel et al., Journal of Leukocyte Biology 2017.