Published: Roussel et al., Methods in Molecular Biology 2019

Congrats to Mikael Roussel and Todd Bartkowiak on their publication of a review and protocol for polarization of human monocytes and assessment by mass cytometry.

  1. Roussel M, Bartkowiak T, Irish JM. Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry. Methods in Molecular Biology 2019 1989:217-226. doi: 10.1007/978-1-4939-9454-0_14.

► This peer-reviewed publication provides a detailed protocol for 1) polarizing human monocytes into different subtypes and 2) high dimensional analysis of the resulting cells by mass cytometry.  Polarized cell types include M1 (M_LPS, M_IFNg), M2 (M_IL-4, M_IL-10), and myeloid derived suppressor cells (MDSCs).  Based on Roussel et al., Journal of Leukocyte Biology 2017.

Roussel et al., MiMB 2019

Roussel et al., MiMB 2019

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