Members

Irish Lab at Vanderbilt 2016

Irish Lab at Vanderbilt 2015

Left to Right: Deon Doxie, Mikael Roussel, Kirsten Diggins, Cara Wogsland, Jonathan Irish, Brent Ferrell, Nalin Leelatian, Hannah Polikowsky, and Allison Greenplate


Current Irish Lab Members


Jonathan M. Irish, Ph.D.

Jonathan M. Irish, Ph.D.

Jonathan M. Irish, Ph.D.

B.S. in Biology, B.S. Chem. in Chemistry, B.S. in Biochemistry, University of Michigan Ph.D. in Cancer Biology, Stanford University.

Jonathan started the Irish lab at Vanderbilt in December 2011 after finishing his training with Garry Nolan (Ph.D.) and Ron Levy (Postdoc) at Stanford.

Publications: Google Scholar | Pubmed orcid.org/0000-0001-9428-8866

In addition to the individual fellowships listed below, the Irish lab has been supported by NIH/NCI K99/R00 CA143231, Team Chad and the Hematology Helping Hands Fund, the Vanderbilt-Ingram Cancer Center (VICC) P30 CA68485, and the VICC Ambassadors.  Thank you!


 

Brent Cameron, M.D., Ph.D.

Brent Cameron, M.D., Ph.D.

B.S. in Chemistry and Biology from Pittsburg State University.  M.D. and Ph.D. in Neurosciences from Case Western Reserve University.  Brent is Resident in the Radiation Oncology Department at Vanderbilt University Medical Center.

Brent’s project focuses on brain tumor immunology and radiation responses in collaboration with the Ihrie lab.

Publications: Pubmed.


Kirsten Diggins

Kirsten Diggins, Ph.D.

Kirsten Diggins, Ph.D.

B.S. in Microbiology, University of Alabama. Ph.D. in Caner Biology from Vanderbilt University. Kirsten is a Postdoctoral Researcher in the Department of Cancer Biology.

Kirsten’s project uses computational biology tools to identify and characterize human cells.

Publications: Google Scholar | Pubmed orcid.org/0000-0003-1622-0158.

Kirsten’s 2015 paper in Methods described a bioinformatics workflow for mass cytometry (CyTOF) that is now widely used in our lab and beyond:

Methods for discovery and characterization of cell subsets in high dimensional mass cytometry data. Diggins KE, Ferrell PB, Irish JM, Methods. 2015. PubmedDOI2015-05 Diggins et al graphical abstract

Kirsten’s 2017 Nature Methods paper created a new tool to objectively identify cells called Marker Enrichment Modeling (MEM).

Characterizing cell subsets using marker enrichment modeling. Diggins KE, Greenplate AR, Leelatian N, Wogsland CE, Irish JM, Nature Methods. 2017. PubmedDOI.

Trivia: Kirsten’s MEM paper coined the term ‘cytotype’ to describe the identity of a cell subset.  In the word cytotype, ‘cyto’ means cell and ‘type’ means identity or grouping.  MEM enables automated cytotyping in a cytomic study (e.g. mapping all the cell types in a tissue or tumor) the way other tools enable genotyping in a genomic study (e.g. mapping all the genetic elements in a genome).

Marker Enrichment Modeling (MEM) code is available for academic and non-profit use and can be licensed for commercial use through VUeInnovations: https://mem.vueinnovations.com/

Diggins et al - Nature Methods 2017 MEM Fig 1

 

This paper by Diggins et al. in Nature Methods 2017 entitled, “Characterizing cell subsets using marker enrichment modeling,” was recently featured:

Investigators seek new way to define cell identity

 


Deon Doxie

Deon Doxie

Deon Doxie

B.S. in Chemistry, University of South Carolina. Deon is in the Vanderbilt Cancer Biology Ph.D. training program.

Deon’s project focuses on high content single cell analysis of melanoma tumors.

Publications: Google Scholar | Pubmed orcid.org/0000-0002-5383-0441

Deon’s Ph.D. training was supported by NIH/NCI T32 CA009592 training grant Microenvironmental Influences in Cancer and by R25 GM062459 the Initiative for Maximizing Student Development (IMSD).

Deon’s book chapter in Current Topics in Microbiology & Immunology: Doxie DB and Irish JM.  High-Dimensional Single-Cell Cancer Biology Current Topics in Microbiology & Immunology 2014 Mar 27. PMC4216808PubmedDOI. 2014 Doxie and Irish - High Dimensional Single Cell Cancer Biology

Deon and Nalin’s paper in Cytometry B: Single cell analysis of human tissues and solid tumors with mass cytometry. Leelatian N*, Doxie DB*, Greenplate AR, Mobley BC, Lehman JM, Sinnaeve J, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM, Cytometry B. 2016. PubmedDOI. *denotes equal contribution.

Leelatian and Doxe et al - Cytometry B 2016 Melanoma Fig 1

This paper by Leelatian and Doxie et al., Cytometry B, entitled, “Single cell analysis of human tissues and solid tumors with mass cytometry” was recently featured:

Single-cell analysis of solid tumors


 

P Brent Ferrell

P. Brent Ferrell, M.D.

P. Brent Ferrell, M.D.

B.A., Davidson College M.D., University of North Carolina, Chapel Hill.

Brent is Instructor in the Department of Medicine and a member of Vanderbilt’s Tinsley R. Harrison Society physician-scientist training program.

Brent’s project uses single cell tools to characterize how acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) cells differ in phenotype, signaling, and treatment response from non-cancer stem cells and differentiated myeloid cells.

Brent’s Fellowship has been supported by the Tinsley R. Harrison Society and the NIH Paul Calabresi K12 CA090625 training grant via the Vanderbilt Clinical Oncology Research Career Development Program (VCORCDP).

Publications: Google Scholar | Pubmed orcid.org/0000-0003-1140-9154.

Brent’s 2016 paper in PLoS One is the first mass cytometry (CyTOF) study of initial chemotherapy treatment response in AML and showed that standard therapies fail not because they select for a stem-like cell, but because the leukemia cells adopt an abnormal phenotype that might represent a new treatment target: 2016 Ferrell et al. - PLOS ONE - High-Dimensional Analysis of Acute Myeloid Leukemia Reveals Phenotypic Changes in Persistent Cells during Induction Therapy High-Dimensional Analysis of Acute Myeloid Leukemia Reveals Phenotypic Changes in Persistent Cells during Induction Therapy. Ferrell PB Jr, Diggins KE, Polikowsky HG, Mohan SR, Seegmiller AC, Irish JM. PLoS One. 2016


 

Allison Greenplate

Allison Greenplate

B.S. in Biology & Templeton Honors College, Eastern University Allie is in the Vanderbilt Microbiology & Immunology Ph.D. training program.  Her project focuses on cancer immunotherapy and immune cell signaling. Allie’s training is supported by NIH/NCI F31 CA199993. Publications: Google Scholar | Pubmed orcid.org/0000-0002-2614-3072

Allie’s 2016 paper in Cancer Immunology Research: Greenplate et al - CIR 2016 - High-Dimensional Analysis of Acute Myeloid Leukemia Reveals Phenotypic Changes in Persistent Cells during Induction Therapy Myelodysplastic Syndrome Revealed by Systems Immunology in a Melanoma Patient Undergoing Anti-PD-1 Therapy. Greenplate AR, Johnson DB, Roussel M, Savona MR, Sosman JA, Puzanov I, Ferrell PB, Irish JM. Cancer Immunology Research 2016.

Allie’s 2016 paper in European Journal of Cancer: Greenplate et al - EJC 2016 - Systems Immune Monitoring in Cancer Therapy Systems immune monitoring in cancer therapy. Greenplate AR, Johnson DB, Ferrell PB Jr., Irish JM, European Journal of Cancer 2016.


 

Faustine L’homme, M.D.

Faustine is spending part of a year in the Irish lab studying lymphoma tumor immunology.  She received competitive funding for a “research year” in the lab from Central Hospital, University of Rennes (CHU) in Rennes, France. Faustine is a 4th year hematology resident working towards a specialization in hematology and her home institution is CHU in Rennes, France.  At CHU Rennes, Faustine works with Mikael Roussel, who previously spent 1 year in the lab.


Nalin Leelatian

Nalin Leelatian

Nalin Leelatian, M.D.

M.D., Clinical Medical Science, and Pathology Degrees, Siriraj Hospital, Mahidol University Nalin is in the Vanderbilt Cancer Biology Ph.D. training program.  Her project focuses on signaling, heterogeneity, and cellular identity in human brain tumors. Nalin has been supported by the Vanderbilt International Scholars Program (VISP). Publications: Google Scholar | Pubmed orcid.org/0000-0003-4284-8272   Nalin’s recent paper in Methods in Molecular Biology: Characterizing phenotypes and signaling networks of single human cells by mass cytometry. Leelatian N, Diggins KE, Irish JM, Methods Mol Bio. 2015. PubmedDOI. Nalin’s recent paper in Cancer Cell: Leelatian and Ihrie - Cancer Cell 2016 - Head of the Class: OLIG2 and Glioblastoma Phenotype Head of the Class: OLIG2 and Glioblastoma Phenotype. Leelatian N and Ihrie RA. Cancer Cell 2016.

 Nalin and Deon’s paper in Cytometry B: Single cell analysis of human tissues and solid tumors with mass cytometry. Leelatian N*, Doxie DB*, Greenplate AR, Mobley BC, Lehman JM, Sinnaeve J, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM, Cytometry B. 2016. PubmedDOI*equal contribution

Leelatian and Doxie et al - Cytometry B 2016 Glioma Fig 4

Caroline Maier

Caroline Maier B.S. in Biology, University of Minnesota

Caroline is Research Assistant III in the Irish lab.  Caroline has expertise in phospho-specific flow cytometry (phospho-flow), microscopy, human and mouse tissue preparation, and mass cytometry (CyTOF) and uses these skills to assist Irish lab members across multiple projects. Caroline also assists collaborators on pilot cytometry studies and works with new lab members. Publications: Google ScholarPubmed orcid.org/0000-0003-4680-9553


 

Hannah Polikowsky

Hannah Polikowsky

Hannah Polikowsky

B.S. in Biology, University of Minnesota Hannah is Program Manager of Human Tissue Research in the Irish lab.  Hannah is focused on clinical translational applications of high dimensional single cell cytometry. Hannah also leads mass cytometry (CyTOF) pilot program planning with collaborators for clinical and basic research studies. Publications: Google ScholarPubmed orcid.org/0000-0001-7226-9752   Hannah’s recent paper in Journal of Immunology: Polikowsky et al. - Journal of Immunology - 2016 - Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B Cells Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B cells. Polikowsky HG, Wogsland CE, Diggins KE, Huse K, Irish JM, J Immunol.2015. PubmedDOI.


 

Cara Wogsland

Cara Wogsland

Cara Wogsland

B.S. in Biochemistry, University of Nebraska-Lincoln Cara is in the Vanderbilt Microbiology & Immunology Ph.D. training program.  Her project focuses on signaling control of B cell identity in healthy germinal center reactions and food allergies. Publications: Google Scholar | Pubmed orcid.org/0000-0003-1797-7902


Alumni


 

Kanutte Huse, Ph.D.

Kanutte Huse, Ph.D.

Ph.D., University of Oslo Kanutte spent a year in the Irish lab from August 2013 to August 2014 training in advanced mass cytometry (CyTOF) and studying healthy and lymphoma B cell signaling. Kanutte is currently working as a Postdoctoral Researcher at the University of Oslo on B cell signaling and identity in the germinal center and cancer. Publications: Google Scholar orcid.org/0000-0001-9128-670X


 

Mikael Roussel

Mikael Roussel, M.D., Ph.D.

Mikael Roussel, M.D., Ph.D.

Mikael spent one year in the Irish lab from August 2014 to August 2015 training in advanced mass cytometry (CyTOF) and studying regulatory myeloid and B lineage cells in the context of lymphoma and other hematological disorders. Mikael is an M.D., Ph.D. clinical biologist specialized in Cellular Hematology and his home institution is the University Hospital of Rennes, France. Publications: Google ScholarPubmed | orcid.org/0000-0002-9741-0668   Mikael’s recent paper in The Human Innate Immunity Handbook: Roussel et al - 2016 Handbook of Human Innate Immunity - Dissecting Complex Cellular Systems with High Dimensional Single Cell Mass Cytometry – Dissecting Complex Cellular Systems with High Dimensional Single Cell Mass Cytometry. Roussel M, Greenplate AR, Irish JM, The Human Innate Immunity Handbook 2016


Past Lab Photos


Irish lab at Vanderbilt, July 2014

Irish lab at Vanderbilt, July 2014
Left to Right: Deon Doxie, Brent Ferrell, Jonathan Irish, Hannah Polikowsky, Kirsten Diggins, Cara Wogsland, Kanutte Huse, Allison Greenplate, and Nalin Leelatian

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