Nalin Leelatian, M.D., joined the Irish lab in 2013 as graduate student in Cancer Biology as part of the Vanderbilt International Scholars Program (VISP).
Degrees: Prior to joining the lab, Nalin trained as an M.D., obtained a diploma in Clinical Medical Sciences, and then trained as a pathologist during her residency at Siriraj Hospital, Mahidol University, Thailand. She is training in the Cancer Biology Ph.D. program at Vanderbilt University.
Nalin’s project focuses on signaling, heterogeneity, and cellular identity in human brain tumors.
Nalin was supported by the Vanderbilt International Scholars Program (VISP), her paper in Cytometry B received the Editor’s Best selection for 2017, her Society for Neuro-Oncology 2016 abstract was selected for an oral presentation, and she was selected an AACR Brain Tumor Association Scholar in Training award for her AACR 2017 abstract.
Nalin’s protocol in Methods in Molecular Biology includes isolating peripheral blood mononuclear cells (PBMC), cryopreserving live PMBC, staining cells for surface mass cytometry, and measuring signaling by phospho-specific mass cytometry (phospho-flow). All of the examples are human immune cells:
Leelatian N, Diggins KE, Irish JM. Characterizing phenotypes and signaling networks of human cells by mass cytometry. Methods in Molecular Biology 2015. PMC4656023. Pubmed. DOI.
She wrote a review in Cancer Cell with her co-mentor, Dr. Rebecca Ihrie:
Head of the Class: OLIG2 and Glioblastoma Phenotype. Leelatian N and Ihrie RA. Cancer Cell 2016.
Nalin and Deon’s peer-reviewed methods paper in Cytometry B was the first published application of flow-based mass cytometry to solid human tissues. This study validated mass cytometry for human solid tumors, including glioblastoma, melanoma, and patient derived xenografts (PDX) of small cell lung cancer.
Leelatian N*, Doxie DB*, Greenplate AR, Lehman JM, Sinnaeve J, Mobley BC, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM. Single cell analysis of human tissues and solid tumors with mass cytometry. Cytometry B. PMC5459378. Pubmed. DOI.
This paper was recently selected as the “Editor’s Best” pick for 2017 — congrats Nalin and Deon! A detailed protocol for the methods established here was published in Leelatian et al., Current Protocols 2017.
Nalin also wrote a detailed protocol for human tissue and solid tumor single cell work:
Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA*, Irish JM*. Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis. Current Protocols in Molecular Biology 2017 Apr 3;118:25C.1.1-25C.1.23. PMC5518778. Pubmed. DOI.