Deon Doxie joined the Irish lab at Vanderbilt in 2012 for his Ph.D. thesis research in the Program in Cancer Biology. Deon’s project focuses on high content single cell analysis of melanoma tumors.
Degrees: B.S. in Chemistry, University of South Carolina. Training in the Vanderbilt Cancer Biology Ph.D. training program.
Deon’s book chapter in Current Topics in Microbiology & Immunology:
►This peer-reviewed review discussed how to measure cancer hallmarks in individual cells with an emphasis on quantifying altered cell signaling. It updated Irish et al., Nature Reviews Cancer 2006 and Krutzik et al., Clinical Immunology 2004 to include solid tumors & mass cytometry.
Deon’s co-first author paper in Cytometry B:
Leelatian N*, Doxie DB*, Greenplate AR, Lehman JM, Sinnaeve J, Mobley BC, Kauffmann RM, Werkhaven JA, Mistry AM, Weaver KD, Thompson RC, Massion PP, Hooks MA, Kelley MC, Chambless LB, Ihrie RA, Irish JM. Single cell analysis of human tissues and solid tumors with mass cytometry. Cytometry B. PMC5459378. Pubmed. DOI. *equal contribution, co-first authors.
► This peer-reviewed methods research was conducted in collaboration with Rebecca Ihrie and was the first published application of flow-based mass cytometry to solid human tissues. This study validated mass cytometry for human solid tumors, including glioblastoma, melanoma, and patient derived xenografts (PDX) of small cell lung cancer. This paper was recently selected as the “Editor’s Best” pick for 2017 — congrats Deon and Nalin!
If you’re thinking about doing any single cell work with solid tumors, a detailed protocol for the methods established here was published in Leelatian et al., Current Protocols 2017. This protocol works with scRNA-seq, PDX, phospho-flow, and other approaches (in addition to mass cytometry).
In addition to the first-author papers above and work in review, Deon also co-authored the following at Vanderbilt:
Johnson DB, Estrada MV, Salgado R, Sanchez V, Doxie DB, Opalenik SR, Johnson AS, Greenplate AR, Sanders ME, Lovly CM, Frederick DT, Kelley MC, Irish JM, Shyr Y, Sullivan RJ, Puzanov I, Sosman JA, Balko JM. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nature Communications PMC4740184. Pubmed. DOI.
Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA*, Irish JM*. Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis. Current Protocols in Molecular Biology 2017 Apr 3;118:25C.1.1-25C.1.23. PMC5518778. Pubmed. DOI.