{"id":1552,"date":"2019-05-11T13:46:26","date_gmt":"2019-05-11T18:46:26","guid":{"rendered":"http:\/\/my.vanderbilt.edu\/irishlab\/?p=1552"},"modified":"2019-07-12T15:07:35","modified_gmt":"2019-07-12T20:07:35","slug":"published-roussel-et-al-methods-in-molecular-biology-2019","status":"publish","type":"post","link":"https:\/\/my.vanderbilt.edu\/irishlab\/2019\/05\/published-roussel-et-al-methods-in-molecular-biology-2019\/","title":{"rendered":"Published: Roussel et al., Methods in Molecular Biology 2019"},"content":{"rendered":"<p>Congrats to Mikael Roussel and Todd Bartkowiak on their publication of a review and protocol for polarization of human\u00a0monocytes and assessment by mass cytometry.<\/p>\n<ol>\n<li>Roussel M, Bartkowiak T, <strong><u>Irish JM<\/u><\/strong>. <a href=\"https:\/\/link.springer.com\/protocol\/10.1007\/978-1-4939-9454-0_14\">Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry<\/a>. <strong>Methods in Molecular Biology <\/strong>2019 1989:217-226. doi: 10.1007\/978-1-4939-9454-0_14.<\/li>\n<\/ol>\n<p>\u25ba This peer-reviewed publication provides a detailed protocol for 1) polarizing human monocytes into different subtypes and 2) high dimensional analysis of the resulting cells by mass cytometry.\u00a0 Polarized cell types include M1 (M_LPS, M_IFNg), M2 (M_IL-4, M_IL-10), and myeloid derived suppressor cells (MDSCs).\u00a0 Based on Roussel et al., <em>Journal of Leukocyte Biology<\/em> 2017.<\/p>\n<div id=\"attachment_1556\" style=\"width: 2285px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/cdn.vanderbilt.edu\/t2-my\/my-prd\/wp-content\/uploads\/sites\/424\/2019\/05\/Roussel-et-al.-MiMB-2019.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1556\" class=\"size-full wp-image-1556\" src=\"https:\/\/cdn.vanderbilt.edu\/t2-my\/my-prd\/wp-content\/uploads\/sites\/424\/2019\/05\/Roussel-et-al.-MiMB-2019.jpg\" alt=\"Roussel et al., MiMB 2019\" width=\"2275\" height=\"2323\" srcset=\"https:\/\/cdn.vanderbilt.edu\/t2-my\/my-prd\/wp-content\/uploads\/sites\/424\/2019\/05\/Roussel-et-al.-MiMB-2019.jpg 2275w, https:\/\/cdn.vanderbilt.edu\/t2-my\/my-prd\/wp-content\/uploads\/sites\/424\/2019\/05\/Roussel-et-al.-MiMB-2019-294x300.jpg 294w, https:\/\/cdn.vanderbilt.edu\/t2-my\/my-prd\/wp-content\/uploads\/sites\/424\/2019\/05\/Roussel-et-al.-MiMB-2019-768x784.jpg 768w, https:\/\/cdn.vanderbilt.edu\/t2-my\/my-prd\/wp-content\/uploads\/sites\/424\/2019\/05\/Roussel-et-al.-MiMB-2019-637x650.jpg 637w\" sizes=\"auto, (max-width: 2275px) 100vw, 2275px\" \/><\/a><p id=\"caption-attachment-1556\" class=\"wp-caption-text\">Roussel et al., MiMB 2019<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Congrats to Mikael Roussel and Todd Bartkowiak on their publication of a review and protocol for polarization of human\u00a0monocytes and assessment by mass cytometry. Roussel M, Bartkowiak T, Irish JM. Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry. &hellip; <a href=\"https:\/\/my.vanderbilt.edu\/irishlab\/2019\/05\/published-roussel-et-al-methods-in-molecular-biology-2019\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":565,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"tags":[],"class_list":["post-1552","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/posts\/1552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/users\/565"}],"replies":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/comments?post=1552"}],"version-history":[{"count":2,"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/posts\/1552\/revisions"}],"predecessor-version":[{"id":1557,"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/posts\/1552\/revisions\/1557"}],"wp:attachment":[{"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/media?parent=1552"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/categories?post=1552"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/irishlab\/wp-json\/wp\/v2\/tags?post=1552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}