{"id":459,"date":"2018-04-12T08:34:22","date_gmt":"2018-04-12T13:34:22","guid":{"rendered":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/?page_id=459"},"modified":"2018-04-12T08:36:33","modified_gmt":"2018-04-12T13:36:33","slug":"aryel-furman","status":"publish","type":"page","link":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/trainees\/summer-immersion-2018\/aryel-furman\/","title":{"rendered":"Aryel Furman"},"content":{"rendered":"<p><em><strong>HDX-MS\u00a0 Studies of Integral Membrane Proteins<br \/>\n<\/strong><\/em><\/p>\n<p><strong>Project Abstract<\/strong><\/p>\n<p>The goal of this project is to develop methods for HDX-MS experiments on integral membrane proteins. An abundant, accessible integral membrane protein, ocular lens aquaporin-0, will be used as the model system to optimize sample preparation conditions, digestion conditions, and data acquisition methods for HDX-MS. Bovine lens AQP0 will be purified by anion exchange chromatography and subjected to HDX-MS as a function of exchange time to determine regions of highest exposure and whether membrane spanning regions undergo exchange. With optimized HDX-MS methods, studies of AQP0 structural perturbations and protein-protein interactions will be examined. Of high interest is the effect of cholesterol on AQP0 structure and the interaction of AQP0 with calmodulin.<\/p>\n<p><strong>Training Plan<\/strong><\/p>\n<p>Ary Furman will work closely with Marcus Nagel in the Schey lab to learn the HDX-MS protocol. He will also work with members of the Schey to purify bovine lens AQP0; a routine procedure in the Schey lab. He will attend and present progress reports at the weekly Schey lab meetings and will attend monthly joint Schey\/Meiler\/Crowe lab meetings to learn about the role of HDX-MS in structural biology. Ary will also work with Marcus Nagel to learn protein structure visualization tools and membrane protein modeling tools in order to place his HDX-MS work in a structural biology context. He will present his work at the VSURP poster session at the end of the summer. Dr. Schey will supervise all HDX-MS experiments. Dr. Meiler will advise on structural biology and membrane protein modeling aspects of the project.<\/p>\n<p><strong>Mentors<\/strong><\/p>\n<p>Primary: Kevin Schey<\/p>\n<p>Secondary: Jens Meiler<\/p>\n","protected":false},"excerpt":{"rendered":"<p>HDX-MS\u00a0 Studies of Integral Membrane Proteins Project Abstract The goal of this project is to develop methods for HDX-MS experiments on integral membrane proteins. An abundant, accessible integral membrane protein, ocular lens aquaporin-0, will be used as the model system to optimize sample preparation conditions, digestion conditions, and data acquisition methods for HDX-MS. Bovine lens&#8230;<\/p>\n","protected":false},"author":5620,"featured_media":0,"parent":456,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"tags":[],"class_list":["post-459","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/pages\/459","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/users\/5620"}],"replies":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/comments?post=459"}],"version-history":[{"count":3,"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/pages\/459\/revisions"}],"predecessor-version":[{"id":466,"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/pages\/459\/revisions\/466"}],"up":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/pages\/456"}],"wp:attachment":[{"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/media?parent=459"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nextgenvaccines\/wp-json\/wp\/v2\/tags?post=459"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}