{"id":130,"date":"2020-04-19T19:41:40","date_gmt":"2020-04-20T01:41:40","guid":{"rendered":"https:\/\/my.vanderbilt.edu\/nicolas2020\/?page_id=130"},"modified":"2020-04-20T11:31:00","modified_gmt":"2020-04-20T17:31:00","slug":"results","status":"publish","type":"page","link":"https:\/\/my.vanderbilt.edu\/nicolas2020\/results\/","title":{"rendered":"Results"},"content":{"rendered":"

Proof of Concept<\/strong><\/p>\n

UV-Vis-NIR Spectrophotometry Trials<\/em><\/strong><\/p>\n

To calibrate our device, we needed to show lactic acid detection by absorbance using an IR spectrophotometer. Attempts to detect varied concentrations in water and research-grade acetone are seen in figures 1.<\/span><\/p>\n

\"Fig.
Fig. 1: NIR spectra of lactate absorbance in various media
A) Lactate in water, baseline-adjusted
B) Lactate in water, baseline-adjusted, with water absorbance overlayed
C) Lactate in acetone, baseline-adjusted, with acetone absorbance overlayed<\/figcaption><\/figure>\n

Device Verification<\/em><\/strong><\/p>\n

To prove that the LED-photoconductor circuit worked as designed, the two circuits were put close together (<1cm) and the photoconductor output was recorded.\u00a0<\/span>A Fourier transform of the output showed strong signal at the LED pulse frequency as seen in figure 2.<\/span><\/p>\n

\"Fig.
Fig. 2: Fourier Transform of our output signal<\/figcaption><\/figure>\n

Conclusion<\/strong><\/p>\n

In the given time frame, a construct of a novel circuit for detection of lactate was developed. We were able to come to the following conclusions:<\/span><\/p>\n

    \n
  • Our device can detect changes in absorbance, as seen in figure 4.<\/span><\/li>\n
  • Further optimization needs to be done to finetune the spectroscopy for various concentrations of lactate.<\/span><\/li>\n
  • The lactate signal was virtually undetectable relative to water and other hydrogenated solvents (i.e., low signal-to-noise ratio [SNR]).<\/span><\/li>\n
  • Use a deuterated solvent to acquire a better signal and improve SNR.<\/span><\/li>\n<\/ul>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Proof of Concept UV-Vis-NIR Spectrophotometry Trials To calibrate our device, we needed to show lactic acid detection by absorbance using an IR spectrophotometer. Attempts to detect varied concentrations in water and research-grade acetone are seen in figures 1. Device Verification To prove that the LED-photoconductor circuit worked as designed, the two circuits were put close…<\/p>\n","protected":false},"author":8632,"featured_media":0,"parent":0,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"tags":[],"class_list":["post-130","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/pages\/130","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/users\/8632"}],"replies":[{"embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/comments?post=130"}],"version-history":[{"count":11,"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/pages\/130\/revisions"}],"predecessor-version":[{"id":146,"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/pages\/130\/revisions\/146"}],"wp:attachment":[{"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/media?parent=130"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/my.vanderbilt.edu\/nicolas2020\/wp-json\/wp\/v2\/tags?post=130"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}