The enteric nervous system regulates gut reflexes. Our prior data show that enteric glia regulate the activity and survival of enteric neurons in health and disease through mechanisms that involve the release of mediators through connexin-43 (Cx43) hemichannels. Given that immune cells express receptors for glial mediators released via Cx43, we hypothesized that enteroglial signaling involving Cx43 contributes to immune cell recruitment. Cx43 was ablated in glial cells in hGFAP::CreERT2+/–/Cx43f/f mice (TG) and we assessed the effect of acute and chronic inflammation driven by dextran sodium sulfate (DSS).