Inflammation, injury, and infection upregulate intestinal expression of the tryptophan metabolizing enzyme indoleamine 2,3-dioxygenase 1 (IDO1). In most disease models, IDO1 activity dampens inflammatory responses, reduces host injury and promotes recovery. Although epithelial cells are the predominant source of IDO1 during intestinal inflammation recovery and repair, the specific role of epithelial IDO1 has not been examined.