Infections (candidiasis) caused by Candida spp. including C. tropicalis, have dramatically increased worldwide, indicating that this organism is an emerging pathogenic yeast. Our recent studies indicate that C. tropicalis is significantly higher in Crohn’s disease (CD) patients than in their non-disease first-degree relatives. However, the role of C. tropicalis in exacerbating intestinal inflammation in patients with CD has not been fully elucidated. The aim of the present study was to determine the effect(s) of C. tropicalis infection on intestinal inflammation following acute intestinal injury induced by dextran sodium sulfate (DSS) and its effect on modulation of gut microbiome in mice.