Fecal Microbiota Transplantation (FMT) is effective for the treatment of recurrent Clostridium difficile infection, but its efficacy for ulcerative colitis (UC) remains controversial. Ishikawa, et al reported that combination therapy with FMT and antibiotic pretreatment were effective in patients with UC. In addition, in most of the previous reports, FMT solution is usually prepared under aerobic conditions, and anaerobic bacteria might be decreased during this procedure. To improve the outcome of FMT for UC, we performed the repeated FMT using fecal solution prepared under the anaerobic condition following the antibiotic pretreatment.