Deregulation of intestinal microRNAs (miRNAs) have been reported in adult and, in the last years, also in pediatric patients with Crohn’s disease (CD). We performed a literature search and chose 4 miRNAs from five relevant studies as candidates for an independent validation (miR-21-5p, miR-31-5p, miR-146a-5p, miR-155-5p). The goal of this study was to verify deregulated expression of the candidate miRNAs in inflamed terminal ileum of treatment-naïve pediatric patients with CD.