Colonic epithelium forms a selective barrier between lumen and colonic mucosa. The integrity of colonic epithelium is maintained by cell proliferation, migration and expression of cellular junctions. A compromised colonic epithelium contributes to colonic inflammation in IBD patients. Previously, we showed that aftiphilin (AFTPH) is significantly downregulated in colonic biopsies from UC patients and involved in colonic epithelial cell permeability. In this study, we examined the role of AFTPH in cell proliferation and migration in vitro and in colonic inflammation by generation of total AFTPH knock-out mice.