The folate hydrolase gene (FOLH1), which encodes for the enzyme glutamate carboxypeptidase II (GCPII), has been implicated in the pathogenesis of inflammatory bowel disease (IBD) where it exhibits a robust 300-1000% increase in enzymatic activity in intestinal biopsies collected from Crohn’s Disease and Ulcerative Colitis patients. We have previously shown that systemic administration of the GCPII inhibitor 2-PMPA (IC50=300 pM) led to significant improvement in disease activity index in three commonly used murine models of IBD: DSS-colitis, TNBS-colitis and spontaneous colitis of IL10-/- mice.