Subcutaneous (SC) delivery of biologic therapies is typically self-administered via a prefilled syringe or an auto-injector. Auto-injector devices are designed to offer a more convenient and more consistent administration of the drug product. The Subcutaneous Administration Assessment Questionnaire (SQAAQ) is a novel, 12-item, self-administered questionnaire that assesses ease of use of SC delivery devices and patient confidence while using the device to administer an injection of drug. This study aimed to establish evidence of the content validity of the SQAAQ among patients with moderate to severe ulcerative colitis (UC).