Neutrophil (PMN) migration in the intestine is an essential component of host defense and wound repair. However, dysregulated PMN recruitment is a hallmark of chronic active inflammatory bowel disease (IBD) characterized by persistent inflammation. Disease flares in IBD are associated with marked recruitment of PMN into the intestinal mucosa that results in crypt abscess formation and mucosal ulcerations. Despite the strong association with IBD, the process of PMN transepithelial migration (TEpM) represents a significantly understudied area.