The DISINFECTS trial is a prospective randomized trial investigating three methods of reprocessing duodenoscopes: standard high-level disinfection with ortho-phthalaldehyde disinfectant, standard high-level disinfection with a repeated (double) cycle of disinfectant exposure, and standard high-level disinfection followed by ethylene oxide gas sterilization. No significant difference was noted between these reprocessing methods with regard to proportion of duodenoscopes with MDRO contamination, or demonstrating ≥10 CFU or >0 CFU growth of any aerobic bacteria.