Phosphatidylcholines (PCs) are structural and functional constituents of cell membranes. The activity of acyltransferase (LPCAT) is required for addition of polyunsaturated fatty acids to the sn-2 position of PCs, and is therefore required to maintain cell membrane structure and function. LPCAT3 is the most abundant isoform of LPCAT in the small intestine and liver—are important sites of plasma lipoprotein metabolism. We investigated the effects of Lpcat3 disruption on lipid metabolism in mice.