Recent advances in gene editing have enabled us to easily manipulate DNA using CRISPR (clustered regularly interspaced short palindromic repeats)/CAS9 (CRISPR-associated protein 9) technology.1 CRISPR/CAS9 is an endonuclease that exploits the DNA double-strand break repair pathway to cleave DNA at specific DNA target sites.1 These properties have been translated into the ability to rapidly and precisely modify DNA in cells, a technological revolution that is accelerating biological research.2 In vivo RNA interference screening has been used previously in mouse models to identify the genes involved in liver tumorogenesis or in resistance to biotherapy.