CIC Panels

Note: The CIC would especially like to thank the Vanderbilt Center for Immunobiology (VCI) for supporting the development and testing of the first mouse mass cytometry CIC Panel, CIC-005 – Mouse Systems Immune Monitoring (SIM).

The CIC provides mass cytometry staining only with the following validated CIC Panels. Updated 11/2022

  1. CIC-HT-010 – Human T cell emphasis mass cytometry – 39 features
    1. Targets are all human and include: CD45 HI30, Dead Rhodium, CD66b 80H3, CD16 3G8, CD8 RPAT8, CD14 M5E2, CD4 RPA-T4, CD3 UCHT1, CD19 HIB19, CD45RO UCHL1, CD127/IL-7Ra A019D5, CCR5 NP-6G4, CD20 2H7, CD27 L128, CCR4 205410, CD134/OX40 ACT35, ICOS C398.4A, TCRgd 11F2,CXCR3 G025H7, CD137/41BB 4B4-1, CCR7 G043H7, CTLA4 14D3, CRTH2 BM16, CD95 DX2, CD44 BJ18, CD38 HIT2, CD25 2A3, CD45RA HI100, CXCR5 RF8B2, CD57 HCD57, CXCR4 12G5, HLA-DR/MHC II L243, PD-1 EH12.2H7, CD56 CMSSB, Cells Iridium, CD11b ICRF44
    2. Optional intracellular targets: Ki-67 B56, FoxP3 tri-clonal (Kordasti et al., 2016)
    3. Optional metabolism module: CPT1a , ATP5a (replaces CCR5), GRIM19 , GLUT3 (conflicts with FoxP3 tri-clonal), GLUT1 (replaces CRTH2), CYTOC
    4. Open channels for A (6): 142, 145, 146, 153, 154, 155, 160, 165, 168
  2. CIC-HM-011 – Human myeloid cell emphasis mass cytometry – 37 features
    1. Targets are all human and include: CD45 HI30, Dead Rhodium, CD66b 80H3, CD16 3G8, CD8 RPAT8, CD14 M5E2, CD4 RPA-T4, CD3 UCHT1, CD19 HIB19, CD45R0 UCHT1, CD117 104D2, CD64 10.1,  CD164 67D2, CCR2 K036C2, p-STAT5 47, CD123 6H6, CD13 WM15, p-STAT1 58D6, CD163 GHI/61, CD36 5-271, CD86 IT2.2, p-STAT3 4/P-STAT3, CD11c Bu15, CD32 FUN-2, S100A9 MRP-14, CD206 3.29B1.10, CD44 BJ18, CCR7 G043H7, CD33 WM53, CD68 Y1/82A, CXCR4 12G5, HLA-DR/MHC II L243, PD-1 EH12.2H7, CD127/IL-7Ra A019D5, Cells Iridium, CD11b ICRF44
    2. Open channels (4): 142, 144, 145, 147, 160, 163, 165, 168, 172
  3. CIC-HB-012– Human B cell emphasis mass cytometry  – 36 features
    1. Targets are all human and include:CD45 HI30, Dead Rhodium, CD66b 80H3, CD16 3G8, CD8 RPAT8, CD14 M5E2, CD4 RPA-T4, CD3 UCHT1, CD19 HIB19, CD45R0 UCHT1, CD5 UCHT2,  IgD IA6-2, CD20 2H7, CD27 L128,  CD43 84-3C1, IgGy MHL-38, CD21 BL13, CD62L DREG-56, CD36 5-271, CD22 HIB22, IgGk MHK-49, CD79B CB3-1, CD40 5C3, CD44 BJ18, CD38 HIT2, CD24 ML5, CD45RA HI100, CXCR5 RF8B2, IgM MHM-88, CXCR4 12G5, HLA-DR/MHC II L243, PD-1
    2. EH12.2H7,  CD56 CMSSB, Cells Iridium, CD11b ICRF44
    3. Open channels (9): 142, 144, 145, 149, 154, 158, 161, 163, 164, 168,
  4. CIC-GBM-008 – Human glioblastoma mass cytometry – 28 features
    1. Targets are all human and include: CD45 HI30, Dead Rhodium, HLA-ABC/MHC I W3-32, c-CAS3 D3E9, CD117 104D2, CD15, CD31 WM59, CD34, SOX10, SOX2 O30-678, Nestin, CD133, CD171, CD56 B159, PDGFRβ 18A2, S100B 19-S100B, PDGFRα, TUJ1, CD44, MX, CD49F GoH3, EGFR D38B1, GFAP* 1B4, CD90 5E10, HLA-DR/MHC II L243, Histone H3 D1H2, Cells Iridium, CD11b ICRF44
    2. Ask for details on optional metabolism or intracellular signaling modules.
  5. CIC-U54-SCLC-009 – Human small cell lung cancer mass cytometry  – 45 features
    1. Targets are all human and include: CD45/PTPRC HI30, Dead cells (Rhodium), CD66b 80H3, CD16 3G8, CD8 RPAT8, CD14 M5E2, CD4 RPA-T4, CD3 UCHT1, CD19 HIB19, Epcam, c-CAS3 D3E9 (apoptosis), CK8/18, mMHC1, ASCL1, HES1, b-CATENIN, SYP, phospho-STAT6, SOX2 O30-678, NEUROD1, phospho-AKT, SOX1, TTF1/NKX2.1, CD56/NCAM B159, PDGFRβ 18A2, phospho-STAT3, S100B 19-S100B, YAP1, CD66e BSB-13, BMX, CD49F GoH3, Oct3.4, CD44 BJ18, MET D1C2, Ki-67 Ki-67 (proliferation), CD24 ML5, GFAP 1B4, phospho-ERK, phospho-S6, CD90 5E10, HLA-DR/MHC II L243, CD298, c-MYC, Cells Iridium, CD11b ICRF44
    2. Open channels (2):  139, 161; immune markers optional.

For mouse:

  1. CIC-MS-009 – Mouse Systems Immune Monitoring (SIM) – 29 targets
    1. Targets are all mouse and include (27): CD45, Ly6GC, CXCR5, CD11b, CD16/32, CD69, F4/80, MHC I, CD19, CD3e, TER-119, CCR6, TCRgd, CD62L, CD1d, Sca1/Ly-6AE, Nkp46, CD8a, TCRb, NK1.1,  CD44, CD4, CD117, MHC II (I-A/I-E), CD38, B220, CD11c
    2. Other features (2): Dead Cells, All cells (Ir intercalator)
    3. Optional intracellular mouse targets (2): c-CAS3, Foxp3
    4. Open channels (10): 147, 151, 155, 161, 163, 166, 174, 175, 176, 209
  2. CIC-006 – Mouse spleen B cell emphasis – 24 targets
    1. Targets are all mouse and include: CXCR5, ICOS, CD4, CD43, CD45, CD11b, CD19, IgD, IgM, CD3e, CD8a, CD48, CD9, CD23, CD5, CD40, CD1d, CD54, CD21, CD44, CD86, I-A\I-E, CD38, B220
    2. Open channels (11): 141, 144, 155, 156, 164, 165, 166, 167, 169, 170, 173

Custom conjugated reagents may be used in CIC panels following successful Antibody Titration & Validation.