Why Use This Microscope?

The Lattice Light sheet is a custom-built microscope that allows for rapid spatial imaging of the sample’s biology with limited phototoxicity.   It accomplished this through the creation of a lattice pattern of Bessel beams that are swept back and worth to create a sheet of light and the focal plane.  The sample is then moved through the sheet of light at high-speed using a piezo stage resulting in volumetric imaging with approximately 20-fold reduction in phototoxicity compared to spinning disk. More information about the lattice light sheet. Here’s an interactive tool for Lattice on FPbase.

Detector / Cameras:

• Hamamatsu ORCA-Flash4.0 V3

Emission:

• 488nm
• 561nm
• 583nm
• 640nm

Excitation:

• 527/70-25        Single Bandpass for eGFP/mEmerald using only one fluorophore
• 525/50-25        Single Bandpass for eGFP/mEmerald using more than one fluorophore
• 594R-25            Long pass for mCherry (and similar) using only one fluorophore
• 629/56-25        Single Bandpass for mCherry (and similar) using more than one fluorophore
• 680/42-25        Single Bandpass for long reds (AF647 and similar)
• 527/645-25      Dual Bandpass eGFP/mCherry (and similar)

Objective Lenses:

• Emission: 28.6x, 0.66NA, 3.74mm WD Water Immersion Special Optics Custom Objective
• Excitation: 25x, 1.1NA, 2mm WD, Water Immersion, Nikon Instruments

Charges: $32.50 per hour

Note: To use the microscope you must have an active iLab account. Contact Jenny for more information.

Location:

• 704 Light Hall