Why Use This Microscope?

A Scanned Oblique Plane Illumination microscope (SOPi) is custom built microscope that is designed for cells to organisms.  It has approximately 1.2um resolution allowing for a large field of view with reduced phototoxicity.  It accomplishes this through the sending a sheet of light at a 45 degree angle through the objective and then collecting the emission through the same objective. The image is then reimaged using two additional objective lens.   More information about the SOPi via PubMed. Here’s an interactive SOPi setup tool on FPBase

Detector / Cameras:

• Photometrics Prime 95b, back-illuminated sCMOS camera

Emission:

• 488nm
• 561nm
• 640nm

Excitation:

• 527/70        Single Bandpass for eGFP/mEmerald using only one fluorophore
• 525/50        Single Bandpass for eGFP/mEmerald using more than one fluorophore
• 593/LP         Long pass for mCherry (and similar) using only one fluorophore
• 629/56        Single Bandpass for mCherry (and similar) using more than one fluorophore
• 679/41        Single Bandpass for long reds (AF647 and similar)
• 527/645      Dual Bandpass eGFP/mCherry (and similar)

Objective Lenses:

• Olympus 20x NA 1.0 water dipping lens

Charges: $32.50 per hour

Note: To use the microscope you must have an active iLab account and request access to the Cell Imaging Shared Resource in iLab. Reservations are made through our on-line iLab calendar. Contact Kari  or Jenny to arrange training.

Location:

• 704 Light Hall