Leelatian N, Diggins KE, Irish JM. Characterizing phenotypes and signaling networks of human cells by mass cytometry. Methods in Molecular Biology 2015. PMC4656023. Pubmed. DOI.
► This peer-reviewed protocol includes isolating peripheral blood mononuclear cells (PBMC), cryopreserving live PMBC, staining cells for surface mass cytometry, and measuring signaling by phospho-specific mass cytometry (phospho-flow). All of the examples are human immune cells.