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Why Use This Microscope?
The Nikon PRIMO is a Nikon Ti-2 microscope with widefield fluorescence and an Alveole PRIMO attachment. The PRIMO is a micropatterning, hydrogel polymerization and microfabrication device that allows you to create custom made in vitro cellular microenvironments. It uses a digital micromirror device (DMD) technology that illuminates a pattern with UV light at 375nm.The Nikon Ti-2 is equipped with the D-LEDI fluorescent LED light source and Prime BSI Express Scientific CMOS (sCMOS) camera. The PRIMO also has a white LED light source and Nikon DS-Ri-2 color camera for bright field imaging of histology stained samples. The microscope offers a 22mm FOV image.


  • Prime BSI Express Scientific CMOS (sCMOS) camera
  • Nikon DS-Ri2 color camera

Imaging Modes:

  • PRIMO – micropatterning, hydrogel polymerization, and microfabrication
  • Chromogenic microscopy (DAB, H&E, etc)
  • Conventional fluorescence microscope

Excitation Specs:

  • Nikon D-LEDI LED light engine:
    • 385 nm (DAPI)
    • 475 nm (FITC. GFP)
    • 550 nm (TRITC, Cy3)
    • 621 nm (far red, Cy5)

Objective Lenses:

  • 4x Plan Fluor NA 0.13 WD 17.1mm
  • 10x Plan Fluor DIC N1 NA 0.3 WD 16mm
  • 20x Plan Apo λD OFN25 DIC N2 NA 0.8 WD 800um
  • 20x S Plan Fluor ELWD DIC N1 NA 0.45 WD 8.2mm (for PRIMO)
  • 40x Plan Fluor Oil DIC H N2 NA 1.3 WD 240um

Charges: $33.75 per hour
Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar.Contact Kari to arrange training.


  • MCN T-2216