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Why Use This Microscope?
The Radnor-WF is a Nikon TiE microscope with widefield fluorescence. It is equipped with a SpectraIII fluorescent LED light source with 8 individually controlled diodes, and a Hamamatsu ORCA Flash 4.0 CMOS 2048 x 2048 pixel camera. The automated and encoded X/Y stage allows for quick large image tiling of tissue samples.


  • Hamamatsu ORCA Flash 4.0 CMOS monochrome camera

Imaging Modes:

  • Conventional fluorescence microscope
  • Multi-position (x/y)
  • Tile scans
  • High-speed time-lapse

Excitation Specs:

  • Lumencor SpectraIII LED light engine:
    • 365 nm (DAPI)
    • 440 nm (CFP)
    • 488 nm (FITC. GFP)
    • 514 nm (YFP)
    • 561 nm (TRITC, Cy3)
    • 594 nm (mCherry)
    • 640 nm (far red, Cy5)
    • 730 nm (near IR, Cy7)
  • Objective Lenses:

    • 4x Plan Apo D 0.2 NA WD 20mm
    • 10x Plan Apo 0.5 NA WD 4mm
    • 20x Plan Apo 0.8 NA WD 1mm
    • 100xH HP Apo TIRF 1.5 NA WD 120um Oil

    Charges: $33.75 per hour
    Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar.Contact Kari to arrange training.


    • MCN T-2216