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LSM 510

Why Use This Microscope?

This microscope is well suited for viewing samples on sealed, inverted slides or in 35mm dishes with a #1.5 coverglass (Matek) compatible with the inverted format. All slides MUST be sealed, clean and dry prior to placing on the stage.

Imaging Modes:

  • Laser scanning fluorescence and DIC (Nomarski)
  • 3-D “Z-series”
  • Time-series

Fluorescence Filters: (excitation/emission)

  • Blue/Green (457,488,514nm for blue, green, yellow dyes, e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes)
  • Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
  • Red/Far-Red (633 nm for far-red dyes like Cy-5)

Objective Lenses:

  • 5x/.30 Plan-Neofluar
  • 10x / 0.30 Plan Neofluar
  • 20x / 0.75 Plan-Apochromat
  • 40x / 1.30 Plan-NEOFLUAR OIL
  • 63x / 1.40 Plan-APOCHROMAT OIL
  • 100x / 1.30 Plan-NEOFLUAR OIL

Locations

  • MRBIII 4155
  • LH 714

Charges: $44.50 per hour

Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar. Contact Sean to arrange training.

Download / View Quickstart Guide.