Nikon Spinning Disk
Why Use This Microscope?
The spinning disk confocal platform within the Nikon Center of Excellence is outfitted with an array of high-end tools to enable multi-dimensional high-speed acquisition of live cells for extended periods of time. The system incorporates a Yokogawa CSU-X1 spinning disk head, Andor DU-897 EMCCD, high-speed piezo [z] stage, live cell incubator, automated and encoded X/Y stage, a four-line high-power solid state laser launch, and a full complement of objectives. In addition, a dedicated illumination path introduces a dedicated high-power 405nm diode laser directed by a pair of high-speed galvanometer-driven mirrors to enable targeted photo -activation, -switching/conversion, -bleaching, as well as photodamage. Both the piezo [z] stage as well as the emission filter wheel can be “triggered” by the camera, which enables very high speed acquisition of multichannel z-stacks over time.
Camera
- Photometrics Prime 95B sCMOS monochrome camera
Imaging Modes:
- Spinning disk confocal
- High-speed channel switching (multi-channel)
- High-speed multi-plane (“Z-stack”)
- Multi-position (x/y)
- Tile scans
- High-speed time-lapse
- Targeted (ROI-based) photo –activation, -conversion, -bleaching (FRAP), -ablation
Excitation Specs:
- Full set of visual cubes for blue, green, and red –emitting probes.
- Diode Laser Lines and Power (measured at fiber tip):
- 405nm, 15mw (DAPI)
- 488nm, 15mW (FITC, GFP)
- 561nm, 15mW (TRITC, Cy3, mCherry)
- 647nm, 15mW (far-red, Cy5)
- Photo-stimulation Laser
- 405nm, 100mW
Emission Specs:
- Emission filters for spinning disk confocal applications:
- 455nm (+/- 25nm)
- 525nm (+/- 18nm)
- 605nm (+/- 35nm)
- 641nm (+/- 75nm)
- 700nm (+/- 37nm)
- Dual Emission: 525nm (+/- 25nm) and 646 (+/- 66nm)
Objective Lenses:
- Plan Fluor 4x 0.13 NA WD 12.2mm
- Plan Apo Lambda 10x 0.45 NA WD 4.00mm
- Plan Apo Lambda 20x 0.75 NA WD 1.00mm
- Plan Fluor 40x Oil DIC H N2 1.30 NA WD 0.20mm
- Plan Apo Lambda Oil 60x 1.40 NA WD 0.13mm
- Apo TIRF Oil 100x 1.49 NA WD 0.12mm
Charges: $33.75 per hour
Note: To use the microscope you must have an active Account with iLab. Contact Kari to arrange training.
Location
- MCN T-2216 NIKON COE