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Confocal Microscopes

The Vanderbilt Medical Center CISR Facility houses seven confocal microscopes for use in medical research studies by various faculty, staff, and students. Please remember, access to all CISR equipment is limited to registered users only. Please Click Here for more information on the registration process.

Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images. The key feature of confocal microscopy is its ability to produce in-focus images of thick specimens, a process known as optical sectioning. Images are acquired point-by-point and reconstructed with a computer, allowing three-dimensional reconstructions of topologically-complex objects.

In a confocal laser scanning microscope, a laser beam passes through a light source aperture and then is focused by an objective lens into a small (ideally diffraction limited) focal volume within a fluorescent specimen. A mixture of emitted fluorescent light as well as reflected laser light from the illuminated spot is then recollected by the objective lens. A beam splitter separates the light mixture by allowing only the laser light to pass through and reflecting the fluorescent light into the detection apparatus. After passing a pinhole, the fluorescent light is detected by a photodetection device (a photomultiplier tube (PMT) or avalanche photodiode), transforming the light signal into an electrical one that is recorded by a computer.

LSM 880
Olympus FV-1000
LSM 710
LSM 780
Nikon Spinning Disk
LSM 510 META Inverted

 

 

 

 

 

 

 

 

 

 

 

 

 

Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images. The key feature of confocal microscopy is its ability to produce in-focus images of thick specimens, a process known as optical sectioning. Images are acquired point-by-point and reconstructed with a computer, allowing three-dimensional reconstructions of topologically-complex objects.

Check out the table below to compare our available confocal microscopes:scope_compare_table

 

 

 

 

 

 

 

 

 

 

It would also be great if you could image the same cell type/fluors across the different instruments to provide direct comparisons on the different instruments.

Standard confocal microscopy has a resolution limit of 200 nm. AiryScan technology will allow for an increase of resolution up to 120 nm. Check the below image to see the difference in this technology:

Human RPE cells

 

In a confocal laser scanning microscope, a laser beam passes through a light source aperture and then is focused by an objective lens into a small (ideally diffraction limited) focal volume within a fluorescent specimen. A mixture of emitted fluorescent light as well as reflected laser light from the illuminated spot is then recollected by the objective lens. A beam splitter separates the light mixture by allowing only the laser light to pass through and reflecting the fluorescent light into the detection apparatus. After passing a pinhole, the fluorescent light is detected by a photodetection device (a photomultiplier tube (PMT) or avalanche photodiode), transforming the light signal into an electrical one that is recorded by a computer.

 

Please remember, access to all CISR equipment is limited to registered users only. Click Here for more information on the registration process.