Vanderbilt University Logo
Skip to main content

Nikon Spinning Disk

Why Use This Microscope?
 
The spinning disk confocal platform within the Nikon Center of Excellence is outfitted with an array of high-end tools to enable multi-dimensional high-speed acquisition of live cells for extended periods of time. The system incorporates a Yokogawa CSU-X1 spinning disk head, high-speed piezo [z] stage, live cell incubator, automated and encoded X/Y stage, a four-line high-power solid state laser launch, and a full complement of objectives. In addition, a dedicated illumination path introduces a dedicated high-power 405nm diode laser directed by a pair of high-speed galvanometer-driven mirrors to enable targeted photo -activation, -switching/conversion, -bleaching, as well as photodamage. Both the piezo [z] stage as well as the emission filter wheel can be “triggered” by the camera, which enables very high speed acquisition of multichannel z-stacks over time.

Camera

  • Photometrics Prime 95B sCMOS monochrome camera

Imaging Modes:

  • Spinning disk confocal
  • High-speed channel switching (multi-channel)
  • High-speed multi-plane (“Z-stack”)
  • Multi-position (x/y)
  • Tile scans
  • High-speed time-lapse
  • Targeted (ROI-based) photo –activation, -conversion, -bleaching (FRAP), -ablation

Excitation Specs:

  • Full set of visual cubes for blue, green, and red –emitting probes.
  • Diode Laser Lines and Power (measured at fiber tip):
    • 405nm, 15mw (DAPI)
    • 488nm, 15mW (FITC, GFP)
    • 561nm, 15mW (TRITC, Cy3, mCherry)
    • 647nm, 15mW (far-red, Cy5)
  • Photo-stimulation Laser
    • 405nm, 100mW

Emission Specs:

  • Emission filters for spinning disk confocal applications:
    • 455nm (+/- 25nm)
    • 525nm (+/- 18nm)
    • 605nm (+/- 35nm)
    • 641nm (+/- 75nm)
    • 700nm (+/- 37nm)
    • Dual Emission: 525nm (+/- 25nm) and 646 (+/- 66nm)

Objective Lenses:

  • Plan Fluor 4x 0.13 NA WD 12.2mm
  • Plan Apo Lambda 10x 0.45 NA WD 4.00mm
  • Plan Apo Lambda 20x 0.75 NA WD 1.00mm
  • Plan Fluor 40x Oil DIC H N2 1.30 NA WD 0.20mm
  • Plan Apo Lambda Oil 60x 1.40 NA WD 0.13mm
  • Apo TIRF Oil 100x 1.49 NA WD 0.12mm

Charges: $33.75 per hour
Note: To use the microscope you must have an active Account with iLab. Contact Kari to arrange training.

Location

  • MCN T-2216 NIKON COE

Download / View Quickstart Guide.