Olympus FV-1000 Inverted Confocal Microscope
Why Use This Microscope?![](https://my.vanderbilt.edu/cisr/files/2021/12/FV-1000_Drop.jpg)
This microscope is well suited for viewing polarized cells and tissue samples on sealed, inverted slides or in 35mm Matek dishes with a #1.5 coverglass. Imaging through a non-glass surface is discouraged. All slides MUST be sealed, clean and dry prior to placing on the stage.
![](https://my.vanderbilt.edu/cisr/files/2021/12/FV-1000_Drop.jpg)
This microscope is well suited for viewing polarized cells and tissue samples on sealed, inverted slides or in 35mm Matek dishes with a #1.5 coverglass. Imaging through a non-glass surface is discouraged. All slides MUST be sealed, clean and dry prior to placing on the stage.
Imaging Modes:
- Laser scanning fluorescence and DIC (Nomarski)
- 3-D “Z-series”
- Time-series
- Simultaneous bleach/scan for PA-GFP and FRAP
Fluorescence Filters: (excitation/emission)
- NearUV/Blue (405nm for UV dyes e.g, DAPI)
- Blue/Green (457,488,514nm for blue, green, yellow dyes,
e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes) - Green/Red (543nm for Cy-3, Texas Red, or rhodamine-like dyes)
- Red/Far-Red (633 nm for far-red dyes like Cy-5)
Objective Lenses:
- 10x / 0.30 Plan Neofluar
- 20x / 0.80 SPlan-UApo
- 40x / 1.30 Plan-Neofluar OIL
- 60x / 1.45 Plan-Apochromat OIL
- 100x / 1.40 SPlan-UApo OIL
Charges: $49.50 per hour
Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar. Contact Sean to arrange training.
Location
- MRBIV 10434