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Cell Imaging Shared Resource

Zeiss LSM880 with AiryScan

Why Use This Microscope?
 
The Zeiss LSM880 with AiryScan is a state of the art confocal microscope with Super Resolution capability (up to 1.7x increased resolution). In addition to routine confocal imaging, it is equipped with an AiryScan module that allows for maximal sensitivity and resolution. It is also equipped with a Definite Focus module to eliminate thermal drift or focal offset in the Z-dimension.
 
This microscope is well suited for confocal fluorescent imaging of live cell/tissue preparations, viewing samples in culture dishes, culture plates (6 wells and greater), chamber slides that can be inverted and a variety of custom made (by user) chambers no larger than a standard culture plate. The live tissue chamber with atmosphere heater, stage heater, humidity and CO2 control permit long-term time-lapse imaging for a wide variety of applications. This microscope is also suitable for fixed cell/tissue imaging, using culture dishes or slides. Imaging through plastic dishes is limited to 5x, 10x and 20x lens. Higher magnification must be through coverslips on slides or MatTek-type culture dishes. All slides must be sealed, clean and dry prior to placing on the stage.

Imaging Modes:

  • AiryScan for Super Resolution (up to 1.7x increased resolution)
  • Laser scanning fluorescence and DIC (Nomarski)
  • 3-D “Z-series”
  • Time-series
  • Tile scans (mosaics)
  • Co-localization, FRET, FRAP, linear unmixing
  • FCS

FLUORESCENCE EXCITATION/EMISSION

  • 405nm Long UV for dyes such as DAPI, Hoechst 346, fluorogold
  • 458,488, 514nm Blue/Green for blue, green, yellow dyes,
    e.g., CFP,YFP,GFP, Cy-2, FITC-like dyes
  • 561nm Green/Red for Cy-3, m-Cherry, or rhodamine-like dyes)
  • 633 nm Red/Far-Red for far-red dyes like Cy-5, TO-PRO, Alexa 647
  • Emission bandwidth for all dyes/laser lines is continuously variable providing huge flexibility for collecting images of unusual dyes and for minimizing bleed-through between colors.

Objective Lenses:

  • 5x/0.25 Fluor, WD=12.5mm
  • 10x/0.50 Plan-Apochromat, WD=2.0mm
  • 20x/0.80 Plan-Apochromat, WD=0.55mm
  • 40x/1.1 LD C-Apochromat, Water Corr, WD=0.62mm
  • 40x/1.30 C Plan-Apochromat Oil, WD=0.22mm
  • 63x/1.40 Plan-APOCHROMAT OIL, WD=0.19mm

Charges: $49.50 per hour
Note: To use the microscope you must have an active Account with iLab. Contact Tegy, or Jenny, to arrange training.

Location

  • LH 704

Download / View Quickstart Guide.