Marcus Nagel

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Structural Studies of Antibody-Antigen Complexes by HDX and Crosslinking Mass Spectrometry

Project Abstract

Hydrogen Deuterium exchange (HDX) and chemical crosslinking (XL) experiments will be carried out to produce structural data and constraints for molecule modeling of antibody-antigen complexes. These chemical approaches will be combined with mass spectrometry (HPLC-MS/MS) analysis to identify specific sites of interaction in these complexes. These data will feed into the pipeline for structural analysis that includes EM, SAS, and crystallographic data in order to model three-dimensional antibody-antigen complexes and to design improved antibodies and vaccines.

Mentors

Primary: Kevin Schey

Secondary: James E. Crowe, Jr. & Jens Meiler

Type of Trainee

Postdoctoral Fellow