Structural Studies of Antibody-Antigen Complexes by HDX and Crosslinking Mass Spectrometry
Hydrogen Deuterium exchange (HDX) and chemical crosslinking (XL) experiments will be carried out to produce structural data and constraints for molecule modeling of antibody-antigen complexes. These chemical approaches will be combined with mass spectrometry (HPLC-MS/MS) analysis to identify specific sites of interaction in these complexes. These data will feed into the pipeline for structural analysis that includes EM, SAS, and crystallographic data in order to model three-dimensional antibody-antigen complexes and to design improved antibodies and vaccines.
Primary: Kevin Schey
Secondary: James E. Crowe, Jr. & Jens Meiler
Type of Trainee