Protocol PDF Document (version 1.2): cst
Instructions:
- Create two separate axial seed regions (at approx. axial slice 49), one for each side
- Create two separate axial ROI regions (at approx. axial slice 39), one for each side.
- Create one ROA region and draw a sagittal ROA slice at the midline.
- In the region list, check the left seed/ROI regions and ROA region, then perform fiber tracking. Based on this output, the other ROA placements will be clearer.
- Using the same ROA file, draw 1-3 more ROA regions:
- on the coronal slice anterior to the seed region (at approx. coronal slice 107)
- on the coronal slice posterior to the seed region (at approx. coronal slice 137)
- Check only the left seed/ROI region and ROA regions, then perform fiber tracking. Under the tract list, make sure only the desired left tract is checked and highlighted in purple. Save region, tract, and density files.
- Uncheck the left seed/ROI region and check the right seed/ROI region and ROA region, then perform fiber tracking. Under the tract list, make sure only the desired right tract is checked and highlighted in purple. Save region, tract, and density files.
See Wakana et al. or Rheault et al for robust anatomically meaningful dissection protocols.
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